pegfp c1 empty plasmid (Thermo Fisher)
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pegfp c1 empty plasmid
Pegfp C1 Empty Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1+empty+plasmid/pmc09778279-130-27-40
Average 86 stars, based on 1 article reviews
Pegfp C1 Empty Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1+empty+plasmid/pmc09778279-130-27-40
Average 86 stars, based on 1 article reviews
pegfp c1 empty plasmid - by Bioz Stars,
2026-09
86/100 stars
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Generated:Article Title: The HPV-18 E7 CKII phospho acceptor site is required for maintaining the transformed phenotype of cervical tumour-derived cells Article Snippet: The same plasmid was used to generate pCMV HPV-16 E7 S31A/S32A using the forward primer 5’-CTCTACTGTTATGAGCAATTAAATGACGCCGCAGAGGAGGA-3’ and the reverse primer 5’-TCATCCTCCTCCTCTGCGGCGTCATTTAATTGCTCATAACA-3’ using the QuickChange site-directed mutagenesis system (Stratagene) as described previously. .. The pEGFP 18 E7 was generated by PCR amplification and sub-cloning of E7 coding sequences from wildtype CMV 18E7 FLAG plasmid as template using the forward primer 5’- ATGCGAATTCATGCATGGA-3’ and the reverse primer 5’- GCATTCTAGATT ACTGCTG-3’ into EcoRI and XbaI restriction sites of the pCANmyc-EGFP 16E7 (JMB-04093) plasmid (kindly provided by J. Mymryk). Polymerase Chain Reaction:Article Title: The HPV-18 E7 CKII phospho acceptor site is required for maintaining the transformed phenotype of cervical tumour-derived cells Article Snippet: The same plasmid was used to generate pCMV HPV-16 E7 S31A/S32A using the forward primer 5’-CTCTACTGTTATGAGCAATTAAATGACGCCGCAGAGGAGGA-3’ and the reverse primer 5’-TCATCCTCCTCCTCTGCGGCGTCATTTAATTGCTCATAACA-3’ using the QuickChange site-directed mutagenesis system (Stratagene) as described previously. .. The pEGFP 18 E7 was generated by PCR amplification and sub-cloning of E7 coding sequences from wildtype CMV 18E7 FLAG plasmid as template using the forward primer 5’- ATGCGAATTCATGCATGGA-3’ and the reverse primer 5’- GCATTCTAGATT ACTGCTG-3’ into EcoRI and XbaI restriction sites of the pCANmyc-EGFP 16E7 (JMB-04093) plasmid (kindly provided by J. Mymryk). Amplification:Article Title: The HPV-18 E7 CKII phospho acceptor site is required for maintaining the transformed phenotype of cervical tumour-derived cells Article Snippet: The same plasmid was used to generate pCMV HPV-16 E7 S31A/S32A using the forward primer 5’-CTCTACTGTTATGAGCAATTAAATGACGCCGCAGAGGAGGA-3’ and the reverse primer 5’-TCATCCTCCTCCTCTGCGGCGTCATTTAATTGCTCATAACA-3’ using the QuickChange site-directed mutagenesis system (Stratagene) as described previously. .. The pEGFP 18 E7 was generated by PCR amplification and sub-cloning of E7 coding sequences from wildtype CMV 18E7 FLAG plasmid as template using the forward primer 5’- ATGCGAATTCATGCATGGA-3’ and the reverse primer 5’- GCATTCTAGATT ACTGCTG-3’ into EcoRI and XbaI restriction sites of the pCANmyc-EGFP 16E7 (JMB-04093) plasmid (kindly provided by J. Mymryk). Plasmid Preparation:Article Title: The HPV-18 E7 CKII phospho acceptor site is required for maintaining the transformed phenotype of cervical tumour-derived cells Article Snippet: The same plasmid was used to generate pCMV HPV-16 E7 S31A/S32A using the forward primer 5’-CTCTACTGTTATGAGCAATTAAATGACGCCGCAGAGGAGGA-3’ and the reverse primer 5’-TCATCCTCCTCCTCTGCGGCGTCATTTAATTGCTCATAACA-3’ using the QuickChange site-directed mutagenesis system (Stratagene) as described previously. .. The pEGFP 18 E7 was generated by PCR amplification and sub-cloning of E7 coding sequences from wildtype CMV 18E7 FLAG plasmid as template using the forward primer 5’- ATGCGAATTCATGCATGGA-3’ and the reverse primer 5’- GCATTCTAGATT ACTGCTG-3’ into EcoRI and XbaI restriction sites of the pCANmyc-EGFP 16E7 (JMB-04093) plasmid (kindly provided by J. Mymryk). Article Title: Effects of N-Acetylcysteine on the Proliferation, Hormone Secretion Level, and Gene Expression Profiles of Goat Ovarian Granulosa Cells Article Snippet: .. GCs were cultured as previously described, seeded into six-well plates at 10 5 per well, and after adherence, 2.5 ug of the pEGFP-C1-RSPO2 overexpression vector plasmid and Cell Culture:Article Title: Effects of N-Acetylcysteine on the Proliferation, Hormone Secretion Level, and Gene Expression Profiles of Goat Ovarian Granulosa Cells Article Snippet: .. GCs were cultured as previously described, seeded into six-well plates at 10 5 per well, and after adherence, 2.5 ug of the pEGFP-C1-RSPO2 overexpression vector plasmid and Over Expression:Article Title: Effects of N-Acetylcysteine on the Proliferation, Hormone Secretion Level, and Gene Expression Profiles of Goat Ovarian Granulosa Cells Article Snippet: .. GCs were cultured as previously described, seeded into six-well plates at 10 5 per well, and after adherence, 2.5 ug of the pEGFP-C1-RSPO2 overexpression vector plasmid and Transfection:Article Title: Effects of N-Acetylcysteine on the Proliferation, Hormone Secretion Level, and Gene Expression Profiles of Goat Ovarian Granulosa Cells Article Snippet: .. GCs were cultured as previously described, seeded into six-well plates at 10 5 per well, and after adherence, 2.5 ug of the pEGFP-C1-RSPO2 overexpression vector plasmid and |